Publications by authors named "T M Che"
2-deoxy-D-glucose (2DG) is a glucose analog converted to 2-deoxy-D-glucose-6-phosphate (2DG-6P) by hexokinase in glycolysis. While 2DG commonly measures glucose uptake, 2DG-6P detects glucose utilization. Here, we present a protocol to measure glucose utilization in various tissues after entering a mouse's body using radiolabeled 2DG.
View Article and Find Full Text PDFViable but nonculturable state in the zoonotic pathogen induced by low-grade fever temperature and antibiotic treatment.
Front Cell Infect Microbiol
December 2024
The zoonotic pathogen is responsible for diverse human diseases, from mild to life-threatening, but it often eludes detection in culture-based assays. This study investigates the potential of to enter a viable but nonculturable (VBNC) state when exposed to human fever temperature or antibiotics, with this state confirmed by successful resuscitation. Viability was assessed using SYBR Green I/PI staining and propidium monoazide-quantitative polymerase chain reaction (PMA-qPCR), while culturability was determined through colony-forming unit (CFU) counting on blood agar plates.
View Article and Find Full Text PDFArticle Synopsis
- A study was conducted on children with new tic disorders (less than 9 months) to explore the relationship between subcortical brain structure and tic symptoms over time.
- The research involved 187 children, grouping them into those with new tic disorders (NT), tic-free healthy controls (HC), and those with chronic tic disorders/Tourette syndrome (TS), assessing brain scans and tic severity scores.
- Findings revealed distinct structural differences, such as a larger right hippocampus in NT children and specific patterns of brain deformation, which could serve as early indicators of tic disorder outcomes regarding symptom improvement.
Article Synopsis
- Renal ischemia-reperfusion injury (RIRI) significantly contributes to acute kidney injury, particularly affecting kidney transplants, and this study investigates the role of cuproptosis-related genes in RIRI to improve diagnostic methods.
- The research utilized bioinformatics to analyze data from 203 RIRI samples, identifying 18 cuproptosis-related differentially expressed genes (CRDEGs) and examining their connections with immune cells and biological pathways.
- Experimental validation in mouse models and cell cultures confirmed the involvement of these genes, highlighting the potential of the copper chelator D-Penicillamine in offering protection against RIRI-induced kidney damage.
Advance on the effects of algal carotenoids on inflammatory signaling pathways.
Eur J Med Chem
January 2025
Article Synopsis
- Inflammation plays a crucial role in the body's self-protection but can also cause health issues when it becomes excessive; hence, regulating inflammatory pathways and inflammasomes is a key research focus.
- Carotenoids, natural pigments found in algae, are noted for their anti-inflammatory and antioxidant properties and are shown to inhibit various inflammatory pathways and inflammasomes.
- The review investigates how carotenoids interact with inflammatory pathways such as p38 MAPK, IL-6/JAK/STAT3, and PI3K, targeting the NLRP3 inflammasome and suggesting potential anti-inflammatory targets for further study.