Nicotine analysis of commercial tobacco products.

The use of AOAC standard methods for the determination of nicotine in commercial tobacco products is shown to be inappropriate. The methods do not provide for suitable sample preparation of these products for the analysis and, in addition, they yield high results. Evidence is presented that the nicotine or total alkaloids measured includes artifact nicotine.

Modulation of lysyl oxidase substrate specificity by the oleate anion.

Extracts of bovine ligamentum nuchae have been assayed for lysyl oxidase activity using as substrates soluble elastin and soluble collagen labeled with tritiated lysine. The assays were performed in the presence and absence of sodium oleate. At 0.

A copper deficiency in neonatal pigs induced by a high zinc maternal diet.

Because high levels of dietary zinc are known to reduce copper body stores, the objective was to determine if a high zinc maternal diet could induce a copper deficiency in the newborn pig fed a dried skim milk--glucose--starch diet unsupplemented with copper. The offspring of gilts, which were fed 5000 ppm of zinc, were allowed to nurse until 3 to 5 days of age when they were weaned and placed in individual stainless-steel pens. The dietary treatments were 0, 5 and 10 ppm added copper from copper sulfate.