Publications by authors named "T Le Bihan"
Article Synopsis
- The study focuses on the antibody response to the Moderna Spikevax COVID-19 vaccine, highlighting the importance of understanding this immune response to pathogens.
- It introduces a new method that combines mass spectrometry and B-cell sequencing to analyze human plasma-derived polyclonal IgG, addressing limitations in traditional B-cell repertoire sequencing.
- The researchers created 12 recombinant antibodies from their sequencing data, with six showing strong binding affinities and neutralizing capabilities against the target antigen.
Article Synopsis
- The study discusses a new labeling strategy for improving peptide detection in mass spectrometry by modifying peptides at their C-terminal and specific amino acids (Aspartic and Glutamic acid) with arginine methyl ester (R-met).
- This modification enhances peptide fragmentation and helps distinguish isobaric ambiguities, which can complicate protein sequencing.
- The approach is tested across various proteins and proteases, demonstrating its effectiveness in producing clearer mass spectrometry results, particularly when focusing on charge states of +2 or higher for better fragmentation.
Diel regulation of protein levels and protein modification had been less studied than transcript rhythms. Here, we compare transcriptome data under light-dark cycles with partial proteome and phosphoproteome data, assayed using shotgun MS, from the alga Ostreococcus tauri, the smallest free-living eukaryote. A total of 10% of quantified proteins but two-thirds of phosphoproteins were rhythmic.
View Article and Find Full Text PDFNext-generation sequencing (NGS) has revolutionized the way we determine the antibody repertoires encoded by B cells in the blood or lymphoid organs and transformed our understanding of adaptive immune responses in many species. Sheep (Ovis aries) have been widely used as a host for therapeutic antibody production since the early 1980s, however, little is known about their immune repertoires or immunological processes affecting the antibody generation. The objective of this study was to employ NGS for a comprehensive analysis of immunoglobulin heavy and light chain repertoires in four healthy sheep.
View Article and Find Full Text PDFImpaired hepatic glucose and lipid metabolism are hallmarks of type 2 diabetes. Increased sulfide production or sulfide donor compounds may beneficially regulate hepatic metabolism. Disposal of sulfide through the sulfide oxidation pathway (SOP) is critical for maintaining sulfide within a safe physiological range.
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