Publications by authors named "T Laulajainen"
Background: Primary cell cultures have been widely used for research purposes, but kidney glomerular and vascular endothelial cells and, particularly, stable endothelial cell lines from these have been difficult to obtain.
Experimental Design: We used electroporation and lipofectin transfection to introduce various transforming constructs, and direct infection with an oncogenic adeno 31 (Ad31) virus to immortalize cells from freshly isolated rat kidney glomeruli and characterized the cells obtained at their 20th, 40th, 60th, and 80th passage for markers of various glomerular cell types.
Results: Direct infection with an oncogenic adenovirus type 31 (Ad31) resulted in a stable cell line (over 120 passages) morphologically resembling epithelial/endothelial cells line (over 120 passages) morphologically resembling epithelial/endothelial cells.