Publications by authors named "T Kubori"
Article Synopsis
- Rab GTPases are targeted by bacterial pathogens to manipulate membrane trafficking, with specific focus on Rab10's role during infection.
- The study identifies ubiquitin signaling, particularly from SidE and SidC ligases, as essential for the modification and recruitment of Rab10 to bacterial vacuoles.
- MavC functions as a negative regulator, crosslinking ubiquitin to SdcB and inhibiting its action, which leads to the removal of Rab10 from vacuoles during later infection stages.
Mobile genetic elements such as conjugative plasmids play a key role in the acquisition of antibiotic resistance by pathogenic bacteria. Resistance genes on plasmids can be transferred between bacteria using specialized conjugation machinery. , the most common bacterium associated with nosocomial infections, harbors a large conjugative plasmid that encodes a type IV secretion system (T4SS).
View Article and Find Full Text PDFThe extensive cellular signalling events controlled by posttranslational ubiquitination are tightly regulated through the action of specialized proteases termed deubiquitinases. Among them, the OTU family of deubiquitinases can play very specialized roles in the regulation of discrete subtypes of ubiquitin signals that control specific cellular functions. To exert control over host cellular functions, some pathogenic bacteria have usurped the OTU deubiquitinase fold as a secreted virulence factor that interferes with ubiquitination inside infected cells.
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- Ubiquitination's ability to influence various biological processes in eukaryotes is tied to the different types of poly-ubiquitin chains, with K6-linked chains being of particular interest but difficult to study due to overlapping regulatory mechanisms.
- Research has identified LotA, a deubiquitinase from the pathogen Legionella pneumophila, that specifically targets and regulates K6-linked poly-ubiquitin chains, offering a clearer way to explore these signaling pathways.
- The study reveals how LotA not only activates deubiquitination but also possesses a unique adaptive ubiquitin-binding domain that prevents the mismanagement of critical proteins within the Legionella-containing vacuole during bacterial infection.
Synthetic engineering and biological containment of bacteriophages.
Proc Natl Acad Sci U S A
November 2022
The serious threats posed by drug-resistant bacterial infections and recent developments in synthetic biology have fueled a growing interest in genetically engineered phages with therapeutic potential. To date, many investigations on engineered phages have been limited to proof of concept or fundamental studies using phages with relatively small genomes or commercially available "phage display kits". Moreover, safeguards supporting efficient translation for practical use have not been implemented.
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