Common behavioral influences of the ErbB1 ligands transforming growth factor alpha and epiregulin administered to mouse neonates.

Ligands for epidermal growth factor (EGF) receptor (ErbB1), such as EGF, transforming growth factor alpha (TGFalpha), and epiregulin, are enriched in body fluids and blood and regulate development of various peripheral organs. It remains however how such circulating polypeptide growth factors influence brain development and function. Here, we performed peripheral injections of TGFalpha and epiregulin to mouse neonates and evaluated immediate physical and neurochemical development and later behavioral consequences.

Mesenchymal-epithelial interactions involving epiregulin in tuberous sclerosis complex hamartomas.

Patients with tuberous sclerosis complex (TSC) develop hamartomas containing biallelic inactivating mutations in either TSC1 or TSC2, resulting in mammalian target of rapamycin (mTOR) activation. Hamartomas overgrow epithelial and mesenchymal cells in TSC skin. The pathogenetic mechanisms for these changes had not been investigated, and the existence or location of cells with biallelic mutations ("two-hit" cells) was unclear.

Early induction of osteoactivin expression in rat renal tubular epithelial cells after unilateral ureteral obstruction.

In this study, we examined the expression of osteoactivin in the rat kidney after unilateral ureteral obstruction. Male Wistar rats were sacrificed at 6h, and on days 1, 2, 3 and 7 after the obstruction. The renal tubular lumens gradually dilated, and marked interstitial fibrosis was confirmed histologically on day 3 after the obstruction.

Expression and significance of a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-1 in an animal model of renal interstitial fibrosis induced by unilateral ureteral obstruction.

To evaluate the pathological roles of a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-1 in rat renal interstitial fibrosis, we examined the expression, localization and effect on growth of ADAMTS-1 in a normal rat kidney cell line (NRK-49F). Increased ADAMTS-1 mRNA expression was observed in the kidney by in situ hybridization after induction of unilateral ureteral obstruction (UUO) in male Wistar rats, the mRNA was localized in the renal tubular epithelial cells in the outer stripe of the outer medulla in the UUO kidney. On the other hand, no positive signals were observed in the sham-operated-kidney.

Transgenic mice over-expressing dicarbonyl/L-xylulose reductase gene crossed with KK-Ay diabetic model mice: an animal model for the metabolism of renal carbonyl compounds.

Carbonyl compounds in the blood stream tend to accumulate in the kidney of diabetic or end stage renal failure subjects. Previously we isolated cDNA encoding dicarbonyl/L-xylulose reductase (DCXR) from a mouse kidney cDNA library. In the present study, transgenic (Tg) mice were generated to study the functional role of DCXR in the kidney.