Publications by authors named "T Knebelsberger"
This report describe the first application of environmental DNA-metabarcoding approach for the assessment of fish species diversity in two marine protected areas of the North Sea: the Doggerbank and the Sylt Outer Reef. We collected 64 water samples and detected 24 fish species. We discuss qualitative differences between MPAs and compare the results with those obtained from bottom-trawl surveys in the same areas.
View Article and Find Full Text PDFA new species of eelpout genus Pachycara Zugmayer, 1911 is described based on five specimens caught at a depth of 24193275 m along the Central and Southeast Indian Ridges in the Indian Ocean. The specimens were collected during the INDEX cruises in 2016, 2018 and 2019, respectively. The new species is distinguished from its congeners by the following combination of characters: scales and pelvic fins absent; lateral line configuration mediolateral; dorsal fin origin associated with vertebrae 79 with no free predorsal pterygiophores; vertebrae 2728 + 5759 = 8587; dorsal-fin rays 7880, anal-fin rays 5862; pectoral fin rays 1315.
View Article and Find Full Text PDFArticle Synopsis
- Quantifying the spawning biomass of fish is essential for establishing fishing quotas, which relies on accurately identifying fish eggs at the species level.
- Traditional methods like COI barcoding are time-consuming and costly, prompting the exploration of MALDI-TOF MS as a faster, cheaper alternative for species identification.
- Using a MALDI-TOF MS reference library created from previously identified fish eggs, the study achieved a 97.5% accurate identification rate for unknown samples, demonstrating the effectiveness of this method while emphasizing the need for optimizing identification scores in such analyses.
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View Article and Find Full Text PDFWe establish the new approach of environmental DNA (eDNA) analyses for the North Sea. Our study uses a multigene approach, including the mitochondrial cytochrome-c-oxidase subunit I (COI) gene for analyzing species composition and the nuclear hypervariable region V8 of 18S rDNA for analyzing supraspecific biodiversity. A new minibarcode primer (124 bp) was created on the basis of a metazoan COI barcode library with 506 species and tested in silico, in vitro, and in situ.
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