Publications by authors named "T Kinashi"
Article Synopsis
- LFA1 and ICAMs play a crucial role in lymphocyte movement and immune response, with integrins being regulated by their binding affinity and valency.
- The study highlights Rab8, a small GTPase, as essential for transporting and accumulating LFA1 in cell contact areas, particularly through low-affinity signaling.
- Findings illustrate that Rab8 enhances LFA1-ICAM1 interactions and signaling through a mechanism that boosts LFA1 density at adhesion sites, primarily affecting avidity rather than affinity.
A novel Rh-catalyzed one-pot homo-coupling reaction of aryl Grignard reagents was achieved. The reaction with bromobenzenes having an electron-donating group or a halogen substituent gave the corresponding homo-coupling products in good yields, although the reaction using heterocyclic or aliphatic bromides scarcely proceeded. A Rh(III)-bis(aryl) complex, which might be formed from RhCl(PPh) and the aryl Grignard reagents, plays an important role in giving the homo-coupling products in this reaction.
View Article and Find Full Text PDFRap1-GTPase activates integrins and plays an indispensable role in lymphocyte trafficking, but the importance of Rap1 inactivation in this process remains unknown. Here we identified the Rap1-inactivating proteins Rasa3 and Sipa1 as critical regulators of lymphocyte trafficking. The loss of Rasa3 and Sipa1 in T cells induced spontaneous Rap1 activation and adhesion.
View Article and Find Full Text PDFArticle Synopsis
- Lymphocyte migration relies on complex chemokine signals, which are influenced by the cell's structure and polarity, but how this is regulated is not fully understood.
- Research shows that the integrin activator Rap1 plays a crucial role in helping T cells reorganize their internal structure for movement, even without attaching to other surfaces.
- The study also identifies that specific proteins, Rasa3 and Sipa1, usually keep cells unpolarized, and removing these proteins can trigger polarization, hinting that Rap1's activation promotes essential cell shape changes for effective immune response.
Immune responses in humanized mice are generally inefficient without co-transplantation of human thymus or HLA transgenes. Previously, we generated humanized mice via the intra-bone marrow injection of CD133+ cord blood cells into irradiated adult immunodeficient mice (IBMI-huNSG mice), which could mount functional immune responses against HTLV-1, although the underlying mechanisms were still unknown. Here, we investigated thymocyte development in IBMI-huNSG mice, focusing on the roles of human and mouse MHC restriction.
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