[Kinetics of the N,N-dimethyl-2-phenylaziridinium reaction with a muscarinic cholinoreceptor and acetylcholinesterases].

Kinetics of the reaction of N,N-dimethyl-2-phenylaziridinium ions with soluble acetylcholinesterases from cobra venom and electric eel as well as with the membrane-bound acetylcholinesterase and the muscarinic acetylcholine receptor from the cerebral cortex of rat brain was investigated at pH 7,5 and 25 degrees C in 0,15 M phosphate buffer. The inhibition reaction involves the non-covalent binding step followed by the irreversible alkylation step. The spontaneous hydrolysis of the aziridinium compound and the reversible inhibition of the enzymes with the hydrolytic product were taken into account in data treatment.