Cleavage of delta and epsilon tubulin PCR products is observed upon PCR purification.

In this study, we report an unusual phenomenon of the self-cleavage of purified PCR products of codon-optimized delta tubulin ( ) and epsilon tubulin ( ) genes through an unknown mechanism. Our studies revealed that intact PCR products for both these genes could be obtained upon PCR amplification from plasmid templates carrying these genes. However, interestingly, purification of these PCR products led to their cleavage through an unidentified mechanism.

Light intensity and spectral quality modulation for improved growth kinetics and biochemical composition of Chlamydomonas reinhardtii.

Effective strategies to optimize algal growth and lipid productivity are critical for the sustainable production of biomass for various applications. Light management has emerged as a promising approach, but the intricate relationship between light intensity, spectral quality, and algal responses remains poorly understood. This study investigated the effects of different light qualities (blue, red-orange, and white-yellow) and intensities (45-305 μmol/m·s) on Chlamydomonas reinhardtii.

Evaluation of AI Model for Cephalometric Landmark Classification (TG Dental).

The accuracy of cephalometric landmark identification for malocclusion classification is essential for diagnosis and treatment planning. Identifying these landmarks is often complex and time-consuming for orthodontists. An AI model for classification was recently developed.

LED alternating between blue and red-orange light improved the biomass and lipid productivity of Chlamydomonas reinhardtii.

Light management is important for improving algae cultivation, specifically by enhancing the productivity of biomass and valued bioproducts. In this study, we present evidence that alternating blue and red-orange light can improve the algal growth kinetics and lipid production in a photobioreactor. Blue (430-445, 460-470 nm) and red-orange light (580-660 nm) from a LED were set at the light saturation point (B: 65 μmol/ms; RO: 155 μmol/ms) and alternated for the cultivation of the green alga Chlamydomonas reinhardtii.

Interactive effects of light quality and culturing temperature on algal cell size, biomass doubling time, protein content, and carbohydrate content.

Light management strategy can be used to improve algal biomass and nutrient production. However, the response of algal metabolism to different light qualities, especially their interaction with other environmental factors, is not well understood. This study focuses on the interactive effects of light quality and culturing temperature on algal protein content and carbohydrate content of C.