Publications by authors named "T J Stout"
Article Synopsis
- The study investigates the relationship between the post-thaw morphology of cryopreserved equine IVP blastocysts and the likelihood of achieving pregnancy after transfer.
- The findings show that while the biopsy procedure before freezing does not negatively impact pregnancy outcomes, blastocysts with over 10% herniated cells post-thaw are less likely to result in pregnancy.
- Factors such as the interval between ICSI and blastocyst confirmation, and the specific stallion and mare involved, significantly influence pregnancy rates, with later developing embryos showing lower success rates.
Onset and Progression of Disease in Nonhuman Primates With PDE6C Cone Disorder.
Invest Ophthalmol Vis Sci
December 2024
Purpose: The California National Primate Research Center contains a colony of rhesus macaques with a homozygous missense mutation in PDE6C (R565Q) which causes a cone disorder similar to PDE6C achromatopsia in humans. The purposes of this study are to characterize the phenotype in PDE6C macaques in detail to determine the onset of the cone phenotype, the degree to which the phenotype progresses, if heterozygote animals have an intermediate phenotype, and if rod photoreceptor function declines over time.
Methods: We analyzed spectral-domain optical coherence tomography (SD-OCT), fundus autofluorescence (FAF), and electroretinography (ERG) data from 102 eyes of 51 macaques (aged 0.
Article Synopsis
- * In a phase 3 trial involving 325 patients, those taking inavolisib had a median progression-free survival of 15.0 months, significantly better than the 7.3 months for the placebo group, indicating better disease management.
- * The treatment with inavolisib showed promising results with a 58.4% objective response rate; however, there were notable side effects, similar between both groups, including high rates of neutropenia
Article Synopsis
- Researchers are utilizing genetic diversity in rhesus macaques to better understand human diseases by sequencing 374 genes related to retinal and neurodevelopmental disorders in 1,845 macaques.
- They identified over 47,000 genetic variants, many of which are shared with human populations, and developed a machine learning tool that effectively predicts the pathogenicity of these genetic variants.
- The study includes the identification of a specific model for autosomal dominant optic atrophy and features a public website that provides access to genetic data from over 2,000 rhesus macaques.
We recently developed re-differentiated equine oviduct epithelial cell (REOEC) monolayers demonstrating various in vivo morphological characteristics, but lacking secondary ciliation. In this study, we evaluated the effects of fetal bovine serum, reproductive steroid hormones, Wnt- and Notch ligands and inhibitors, and different EOEC seeding densities, in both conventional wells and on microporous membranes, on EOEC morphology and, in particular, secondary ciliation. REOEC monolayers were assessed by confocal microscopy after combined staining of nuclei, cilia, and the cytoskeleton.
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