Current findings and future research avenues in the study of interstitial cystitis.

This article reports the progress made in the last several years of investigations into possible pathogenic processes that lead to the development of interstitial cystitis. The evidence presented here asserts that bladder mucin is functionally intact in interstitial cystitis and that the permeability of the afflicted patient's bladder is not greater than normal.

Effect of chronic neuroleptic treatment on central and peripheral muscarinic receptors.

The regulation of muscarinic acetylcholine receptor (MAChR) subtypes in rat striatum, bladder and heart was examined following a 14-day administration of neuroleptics (clozapine or fluphenazine), anticholinergics (atropine) or a combination of anticholinergics and neuroleptics. Levels of MAChRs were ascertained by the use of immunoprecipitation and radioligand binding. The combined treatment of fluphenazine and atropine produced an increase in all MAChR subtype levels in striatum with m1 receptor levels having the largest increase (270%) from control.

A method for stimulation of cyclic AMP levels in vivo by intracerebral injection in the rat olfactory tubercle.

A method is described for stimulation of cAMP levels in brain by direct injection of dopamine (DA) and other neuroactive substances. Intracerebral microinjection was preceded by intraperitoneal injection of 3-isobutyl-1-methylxanthine (IBMX) to inhibit cyclic nucleotide phosphodiesterase. In vivo adenylate cyclase and phosphodiesterase activities were terminated by focused microwave radiation and the injected tissue assayed for protein and cAMP content.

Aryl sulfatase activity in mouse molar odontogenesis.

Arylsulfatase activity has been studied in the developing molar of the Swiss albino mouse from the lamina stage to the appositional stage. Timed-pregnant Swiss albino mice were utilized in this study. Females were sacrificed by ether anesthesia and fetuses extirpated or newborns anesthetized and decapitated.