1,6-Diphenyl-1,3,5-hexatrine as a reporter of inner spore membrane fluidity in Bacillus subtilis and Alicyclobacillus acidoterrestris.

A method to determine the inner membrane fluidity of spore populations was developed using the hydrophobic probe 1,6-diphenyl-1,3,5-hexatriene (DPH). DPH incorporated into the inner spore membrane of Bacillus subtilis ATCC 6633 and Alicyclobacillus acidoterrestris N1100, during static and dynamic fluidity experiments. The membrane fluidity of A.

The Artisanal Production of Pulque, a Traditional Beverage of the Mexican Highlands.

Pulque is a traditional fermented alcoholic, acidic, viscous drink of the Mexican central highlands. Its production from the "aguamiel" (sap) of agave plants dates back ~1,500 years and continues to be made by artisanal methods. However, the variability of pulque's quality and its instability hamper its widespread distribution and consumption.

Clostridium difficile MazF toxin exhibits selective, not global, mRNA cleavage.

Clostridium difficile is an important, emerging nosocomial pathogen. The transition from harmless colonization to disease is typically preceded by antimicrobial therapy, which alters the balance of the intestinal flora, enabling C. difficile to proliferate in the colon.

The Acid Tolerance Response Alters Membrane Fluidity and Induces Nisin Resistance in Listeria monocytogenes.

The ability of L. monocytogenes cells to adapt to a variety of stressors contributes to its growth in a wide range of foods. The present study examines the effect of acid and of the acid tolerance response (ATR) on membrane fluidity and on the organism's resistance to acid and to the bacteriocin nisin.