A Pool of Bacterium-like Particles Displaying African Swine Fever Virus Antigens Induces Both Humoral and Cellular Immune Responses in Pigs.

Background/objectives: African swine fever (ASF), caused by African swine fever virus (ASFV), poses a significant threat to the global swine industry. This underscores the urgent need for safe and effective ASF vaccines.

Methods: Here, we constructed five bacterium-like particles (BLPs) that each display one of the five ASFV antigens (F317L, H171R, D117L, B602L, and p54) based on the Gram-positive enhancer matrix-protein anchor (GEM-PA) system.

Diversity and Multigene Phylogeny of the Genus (Agaricales, Basidiomycota).

is known as a northern-hemisphere-distributed genus with important economic values, especially in Western China. However, its species diversity in Asia and the phylogeny of this genus have not been critically studied. Based on worldwide sampling and multi-locus DNA sequence data (ITS, LSU, , ), the phylogeny of was reconstructed, and the species diversity in Asia was critically studied on the basis of morphology and phylogeny.

Rubber-like Deep Eutectic Solvent-Assisted Poly(-acryloylglycinamide) Hydrogel for Highly Sensitive Pressure Detecting.

Deep eutectic solvent (DES)-based conductive hydrogels have attracted great interest in the building of flexible electronic devices that can be used to replace conventional temperature-intolerant hydrogels and expensive ionic liquid gels. However, current DES-based conductive hydrogels obtained have limited mechanical strength, high hysteresis, and poor microdeformation sensitivity of the assembled sensors. In this work, a rubber-like conductive hydrogel based on -acryloylglycinamide (NAGA) and DES (acetylcholine chloride/acrylamide) has been synthesized by a one-step method.

Directed evolution of diacylglycerol acyltransferases 2 promotes lipids and triglyceride accumulation.

Triacylglycerol (TAG) is a major component of plant-neutral lipids. Diacylglycerol acyltransferase 2 (DGAT2) plays an important role in plant oil accumulation by catalyzing the final step of the Kennedy pathway. In this study, ten DGAT2 sequences were originating from different oil crops into the TAG-deficient yeast strain H1246, to compare their enzyme activity of oil synthesis and filter out potential amino acid residue sites for directed evolution.

Metabolic changes of thalamus assessed by H-MRS spectroscopy in patients of cervical spondylotic myelopathy following decompression surgery.

Objective: To assess the changes of thalamic metabolites before and after surgery in patients with Cervical Spondylotic Myelopathy (CSM) using Hydrogen Proton Magnetic Resonance Spectroscopy (H-MRS) and to investigate its association with improvement in neurological function.

Methods: Forty-eight CSM patients who underwent cervical decompression surgery from December 2022 to June 2023 were included, and 33 healthy volunteers were recruited. All subjects underwent bilateral thalamic H-MRS scans before the surgical procedure, and subsequently again 6 months later.