Publications by authors named "T J Bushell"
Standing wave (SW) microscopy is a method that uses an interference pattern to excite fluorescence from labelled cellular structures and produces high-resolution images of three-dimensional objects in a two-dimensional dataset. SW microscopy is performed with high-magnification, high-numerical aperture objective lenses, and while this results in high-resolution images, the field of view is very small. Here we report upscaling of this interference imaging method from the microscale to the mesoscale using the Mesolens, which has the unusual combination of a low-magnification and high-numerical aperture.
View Article and Find Full Text PDFArticle Synopsis
- Multi-wavelength standing wave microscopy and interference reflection microscopy utilize optical interference to analyze surface structures, but using multiple wavelengths can complicate the resulting topographical maps.
- A new image processing technique has been developed that significantly reduces the thickness and spacing of antinodal fringes, which enhances the clarity and precision of these topographical maps by up to two times.
- The effectiveness of this method is showcased through tests on both non-biological models and live cell specimens, demonstrating its ability to clarify cellular features and reduce ambiguities in surface topography.
Disorders of the central nervous system (CNS) represent a global health challenge and an increased understanding of the CNS in both physiological and pathophysiological states is essential to tackle the problem. Modelling CNS conditions is difficult, as traditional models fail to recapitulate precise microenvironments and animal models of complex disease often have limited translational validity. Microfluidic and organ-on-chip technologies offer an opportunity to develop more physiologically relevant and complex models of the CNS.
View Article and Find Full Text PDFUnlabelled: Angiotensin II (ATII) was approved for septic or other distributive shock due to its property of increasing blood pressure within 3 hours. Limited data exist regarding its effectiveness when used in real-world clinical practice.
Objectives: This study examined ATII as a third-line vasopressor based on institutional approval.
Rationale: Major depressive disorder (MDD) is a leading cause of disability worldwide but currently prescribed treatments do not adequately ameliorate the disorder in a significant portion of patients. Hence, a better appreciation of its aetiology may lead to the development of novel therapies.
Objectives: In the present study, we have built on our previous findings indicating a role for protease-activated receptor-2 (PAR2) in sickness behaviour to determine whether the PAR2 activator, AC264613, induces behavioural changes similar to those observed in depression-like behaviour.