Gene expression analysis for EPHA2 (EPH receptor A2), EPHB2 (EPH receptor B2), S100A9 (S100 calcium binding protein A9), PBEF(nicotinamide phosphoribosyltransferase), LILRB2 (leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2), PLAUR (plasminogen activator, urokinase receptor), LTB (lymphotoxin beta (TNF superfamily, member 3)), WNT5A (wingless-type MMTV integration site family, member 5A) has been conducted using real-time polymerase chain reaction in specimens affected by psoriasis versus visually intact skin in 18 patients. It was revealed that the expression of the nine examined genes was upregulated in the affected by psoriasis compared to visually intact skin specimens. The highest expression was observed for S100A9, S100AS, PBEF, WNT5A2, and EPHB2 genes.
View Article and Find Full Text PDFThe comparative bioinformatic analysis of psoriasis and Crohn disease pathological processes was carried out using the results of microarray experiments deposited in GEO DataSets database. Several common for both pathologies genes and molecular-genetical processes were found. It is suggested that some transcriptional factors including AP-1 system of transcriptional factors are involved in pathological processes both under psoriasis and Crohn disease.
View Article and Find Full Text PDFPsoriasis is an autoimmune multifactorial dermatological disease frequently occured in European population. As for other multifactorial genetic diseases success in psoriasis treatment may be achieved due to determination of molecular genetic mechanisms of pathogenesis triggering and psoriasis candidate genes and proteins searching as targets for drugs. Using bioinformatic analysis of transcriptome data AP-1 transcriptional factor components were revealed as psoriasis candidate genes.
View Article and Find Full Text PDFUnstimulated human peripheral blood mononuclear cells (MNC) underwent death during incubation in vitro. According to morphological criteria the type of death was identified as apoptosis. There was a good correlation between a fraction of apoptotic cells qualified morphologically, and fraction of "apoptotic" comets.
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