RNA-based sample discrimination and classification can be used to provide biological insights and/or distinguish between clinical groups. However, finding informative differences between sample groups can be challenging due to the multidimensional and noisy nature of sequencing data. Here, we apply a machine learning approach for hierarchical discrimination and classification of samples with high-dimensional miRNA expression data.
View Article and Find Full Text PDFNon-hematopoietic cells are essential contributors to hematopoiesis. However, heterogeneity and spatial organization of these cells in human bone marrow remain largely uncharacterized. We used single-cell RNA sequencing (scRNA-seq) to profile 29,325 non-hematopoietic cells and discovered nine transcriptionally distinct subtypes.
View Article and Find Full Text PDF-associated ulcerative colitis flare is sparsely reported in the literature but is a treatable condition with antibiotics including trimethoprim/sulfamethoxazole (TMP-SMX), fluoroquinolones, and second/third-generation cephalosporins. We report a case of a patient with long-standing ulcerative colitis (UC), who presented with bloody stools, fatigue, and oliguria that did not respond to standard regimen including steroids. The lab finding was significant for leukocytosis and anemia.
View Article and Find Full Text PDFIntroduction The electric pulp tester (EPT) is an extensively used diagnostic tool in endodontics. However, several factors, especially the location and thickness of the tooth structures, such as enamel and dentine, can affect the result of an electric pulp test. Further, these factors also alter the pain threshold, which may lead to an inaccurate diagnosis.
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