Enhanced anammox-mediated nitrogen removal in bioelectrochemical systems at prolonged negative electrode potentials.

Bioelectrochemical anaerobic ammonium oxidation (anammox) systems allow eco-friendly removal of nitrogen from reject wastewater coming from biogas processing as the anammox bacteria have previously shown to have c-type cytochromes acting in the extracellular electron transport (EET) mechanism between the bacteria and electrode. The anammoxosome compartment present in anammox bacteria features a highly curved membrane and contains tubular structures along with electron-dense particles that contain iron, which could enhance the process of EET and enhance nitrogen removal by properly applied potentials. In this study, nitrogen removal was investigated in the electrostimulated anammox nitrogen removal (EANR) cells operated comparatively at open circuit and at applied potentials of - 300 mV, - 500 mV, and - 700 mV vs.

Protocol for rapid and cost-effective extraction of genomic DNA from a wide range of wild yeast species for use in PCR-based applications.

Isolation of amplifiable genomic DNA is a prerequisite for the implementation of PCR-based techniques. Here we present a protocol for isolating the genomic DNA from a variety of wild yeast species. This can be completed in approximately 1 h and does not require sophisticated laboratory equipment.

Loss of Conserved rRNA Modifications in the Peptidyl Transferase Center Leads to Diminished Protein Synthesis and Cell Growth in Budding Yeast.

Ribosomal RNAs (rRNAs) are extensively modified during the transcription and subsequent maturation. Three types of modifications, 2'-O-methylation of ribose moiety, pseudouridylation, and base modifications, are introduced either by a snoRNA-driven mechanism or by stand-alone enzymes. Modified nucleotides are clustered at the functionally important sites, including peptidyl transferase center (PTC).

Controlling Chirogenic Effects in Porphyrin Based Supramolecular Systems: Theoretical Analysis Versus Experimental Observations.

Electronic circular dichroism (ECD) spectroscopy is a widely employed method for studying chiral analysis, requiring the presence of a chromophore close to a chiral centre. Porphyrinoids are found to be one of the best chromophoric systems serving for this purpose and enabling the application of ECD spectroscopy for chirality determination across diverse classes of organic compounds. Consequently, it is crucial to understand the induction mechanisms of ECD in the porphyrin-based complexes.

Richness of yeast community associated with apple fruits in Estonia.

Yeasts are single-celled fungi that are widespread around the globe. They are part of a community of microorganisms that use a wide variety of habitats, including fruit surfaces. This study aimed to characterise the culturable epiphytic yeasts associated with apple fruits.