[Study of conditions for cleaving bovine leukemia virus RNA in vitro using ribozymes directed against long terminal repeats].

Ribozyme has been constructed by the oligonucleotide synthesis technique that is capable of splitting bovine leukemia virus (BLV) RNA-substrate in the R-region (358th nucleotide). Ribozyme has been shown to efficiently split the RNA containing R-region of the virus. The efficiency of ribozyme function depends on conditions of the reaction reaching its maximum at 25 degrees C and pH 7.

[Use of U3 promotor from the LTR region of bovine leukemia virus for expressing genes for antiviral antisense RNAs in cell cultures].

The possibility was studied to use the U3 promoter from LTR region of bovine leukemia virus to control the expression of the antisense RNA genes directed against the BLV genome in cell cultures FLK-BLV, CC81, and HeLa. The genetic engineering constructions were obtained carrying the reporter beta-galactosidase gene and the genome of antisense RNA to R-U5 region of the viral genome under the control of the promoter. The functioning of the viral promoter was studied in three cell lines.

[Genetic engineering in the bacterial synthesis of somatostatin].

Series of recombinant plasmids for expression of the synthetic gene somatostatin-14 (SST) as a fusion protein were obtained. The somatostatin gene was fused to chloramphenicol acetyltransferase (cat) or its deleted variant genes. Both parts of the resultant fusion protein were joined through a Met residue.