Double color in situ hybridization of alpha-satellite chromosome 13, 21 specific cosmid clones for a rapid screening of their specificity.

Double color in situ hybridization was used to determine the specificity of cloned alpha-satellite cosmid DNA sequences belonging to satellite DNA sequences shared by chromosomes 13 and 21. It was determined that cosmid clone cos56D8 is more specific to chromosome 13, cosmid clone cos37E is more specific to chromosome 21 and cosmid clones cosA5130 is specific to both chromosomes 13 and 21. These new cosmid probes could bw useful in the molecular-cytogenetic analysis of trisomic cells at Patau or Down syndromes as well as in analysis of marker chromosomes originated from the chromosomes 13 and 21.

Selective monitoring for a Chernobyl effect on pregnancy outcome in Kiev, 1969-1989.

The aim of this investigation was to determine the frequency of adverse pregnancy outcomes in Kiev during the period surrounding the Chernobyl accident on April 26, 1986. Additional effective equivalent doses resulting from the catastrophic irradiation in 1986-1991 was 8.04 mSv for Kiev inhabitants.

Oncogenic adenovirus as mutagen for chinese hamster cells in vitro.

Oncogenic bovine adenovirus (BAV3) was shown to induce chromosome aberrations and gene mutations to 6-mercaptopurine (6MP) resistance in Chinese hamster cells. BAV3 showed the highest mutagenic effect at the chromosome level 12--24 h postinfection. After 48 h the yield of aberrations dropped to the control level, where it remained after 72 and 96 h.