Prevalence of Bartonella sp. in United States military working dogs with infectious endocarditis: a retrospective case-control study.

Objectives: Bartonella infection has been associated with endocarditis in humans, dogs, cats and cattle. In order to evaluate the importance of this pathogen as a possible source of endocarditis in United States military working dogs (MWDs), we performed a retrospective case-control study on 26 dogs with histological diagnosis of culture negative endocarditis (n = 18), endomyocarditis (n = 5) or endocardiosis (n = 3) and 28 control dogs without any histological cardiac lesions.

Methods: DNA was extracted from paraffin embedded cardiac valves and tissues from case and control dogs and submitted to PCR testing with primers targeting the Bartonella gltA gene.

Seroepidemiology of Bartonella vinsonii subsp berkhoffii exposure among healthy dogs.

Objective: To determine seroprevalence of antibodies to Bartonella vinsonii subsp berkhoffii and risk factors for seropositivity among working dogs owned by the US government.

Design: Cross-sectional study.

Animals: 1,872 dogs.

Evaluation of butylated hydroxytoluene as a cryopreservative added to whole or skim milk diluent for bull semen.

Effect of five concentrations of butylated hydroxytoluene on the percent motility and percent intact acrosomes was evaluated for bull sperm frozen and thawed in whole and skim milk diluents. Twelve ejaculates were frozen in .5-ml straws in moving nitrogen vapor after each ejaculate was split among the five butylated hydroxytoluene treatments within the two diluents.

Cryopreservation of murine embryos with trehalose and glycerol.

Several concentrations of trehalose (0.0, 0.04, 0.