Comparison of three source attribution methods applied to whole genome sequencing data of monophasic and biphasic Typhimurium isolates from the British Isles and Denmark.

Methodologies for source attribution (SA) of foodborne illnesses comprise a rapidly expanding suite of techniques for estimating the most important source or sources of human infection. Recently, the increasing availability of whole genome sequencing (WGS) data for a wide range of bacterial strains has led to the development of novel SA methods. These techniques utilize the unique features of bacterial genomes adapted to different host types and hence offer increased resolution of the outputs.

Genotypic Antimicrobial Resistance Profiles of Diarrheagenic and Nontyphoidal Strains Isolated from Children with Diarrhea and Their Exposure Environments in Ethiopia.

Background: Antimicrobial resistance (AMR) poses a significant global threat, particularly in low- and middle-income countries, such as Ethiopia, where surveillance is limited. This study aimed to predict and characterize the AMR profiles of diarrheagenic (DEC) and nontyphoidal (NTS) strains isolated from human, animal, food, and environmental samples using whole genome sequencing.

Methods: A total of 57 NTS and 50 DEC isolates were sequenced on an Illumina NextSeq 550.

Occurrence and diversity of Campylobacter species in diarrheic children and their exposure environments in Ethiopia.

Campylobacter is a major zoonotic foodborne pathogen that poses a significant public health threat, particularly among children and immunocompromised individuals. However, data on the occurrence and sources of Campylobacter infection remain scarce in Ethiopia. This study assessed the occurrence, diversity, and relationships between Campylobacter from diarrheic children and potential exposure sources using whole-genome sequencing.

Foodborne Bacterial Pathogens in Animal, Food, and Environmental Samples Collected From the Physical Exposure of Children With Diarrhea in Ethiopia: A One Health Approach.

Foodborne pathogens (FBPs) are transmitted principally through the consumption of contaminated food or drinking water and pose a remarkable public health risk, particularly in low-income countries. A cross-sectional study was conducted between November 2021 and January 2023 to estimate the prevalence, co-occurrence, and monthly patterns of FBPs in the physical exposures of children with diarrhea in Harar town and Kersa district, Ethiopia. Animal, food, and environmental samples were collected from direct or indirect contact sites of children with diarrhea.

Risk Factors for Infection in Children under Five Years: A Hospital-Based Study in Kilimanjaro Region, Tanzania.

is among the causative agents for diarrhea worldwide, but its risk factors in Tanzanian children are poorly understood. A hospital-based cross-sectional study was conducted in Moshi, Kilimanjaro region, from July 2020 to November 2022 among children under five admitted with diarrhea. A questionnaire was administered to all parents/caretakers of the enrolled children.