Publications by authors named "T H Kawula"
is a Gram-negative facultative intracellular bacterial pathogen that is classified by the Centers for Disease Control and Prevention as a Tier 1 Select Agent. infection causes the disease tularemia, also known as rabbit fever. Treatment of tularemia is limited to few effective antibiotics which are associated with high relapse rates, toxicity, and potential emergence of antibiotic-resistant strains.
View Article and Find Full Text PDFFrancisella tularensis is a zoonotic, facultative intracellular bacterial pathogen that replicates in a variety of cell types during infection. Following entry into the cell and phagosome escape, the bacterium replicates rapidly in the cytoplasm. F.
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- Merocytophagy is a process where cells "eat" small parts of neighboring cells to acquire microbes and other materials through phagocytosis.
- Researchers developed a synchronized transfer assay to improve the analysis of recipient cells after these transfer events, allowing for high-purity isolation from donor cells.
- This new protocol focuses on studying bacterial infections and cellular processes related to merocytophagy, making it easier to explore cell-to-cell transfer mechanisms.
Article Synopsis
- Phagocytic cells can ingest bacteria from infected cells without killing the original host cell, as shown in earlier research (Steele et al., 2016).
- This study investigates what happens immediately after the bacteria are transferred, finding that they are contained within double-membrane vesicles that contain components from the original infected cell’s plasma membrane.
- The researchers used a specific strain of bacteria to observe that this transfer occurs in the lungs of infected mice, supporting the idea that macrophages can acquire material from other cells through a unique process related to phagocytosis.
is a Gram-negative, facultative, intracellular bacterial pathogen and one of the most virulent organisms known. A hallmark of pathogenesis is the bacterium's ability to replicate to high densities within the cytoplasm of infected cells in over 250 known host species, including humans. This demonstrates that is adept at modulating its metabolism to fluctuating concentrations of host-derived nutrients.
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