Publications by authors named "T H Giddings"
Article Synopsis
- - The study evaluated Project DINE, an initiative in Georgia that added nutrition and breastfeeding education to Healthy Start programs, specifically targeting Black expecting parents using a community-based participatory approach.
- - Results showed significant improvements in self-reported diet quality (p = .025) among 61 couples and an increase in correct breastfeeding knowledge from 78% to 84% (p = .0009) among 89 participants after the intervention.
- - Qualitative feedback highlighted the importance of involving both expecting mothers and fathers in nutrition and breastfeeding education to enhance maternal health outcomes during pregnancy.
Objective: To describe the intervention and research methods of Project Dads in Nutrition Education, an intervention that adds nutrition and breastfeeding education to the existing Healthy Start program in Georgia for Black expecting mothers and fathers to improve nutrition literacy, health outcomes, and breastfeeding rates.
Design: This 2-year community-based participatory research study employs a cluster randomization factorial design.
Setting: Six Healthy Start program sites (3 intervention and 3 control sites) in Georgia counties with high infant and maternal mortality rates.
Article Synopsis
- During sexual reproduction in ciliates like Tetrahymena thermophila, specialized adhesion zones are formed to allow for the exchange of gametic pronuclei through a membrane called the mating junction.
- The passage of pronuclei through this junction requires microtubules and results in two membrane breaches that need to be sealed post-fertilization.
- Rather than simply growing new membrane from the edges like in other cells, the breaches transform into complex membrane structures that grow into the partner's cytoplasm and connect to the plasma membrane, revealing a unique mechanism for membrane restoration.
Building Cell Structures in Three Dimensions: Electron Tomography Methods for Budding Yeast.
Cold Spring Harb Protoc
March 2017
has been an important model system for numerous cellular, genetic, and molecular studies. However, this small eukaryote presents a challenge for imaging at the electron microscope level. Preparation of yeast using high-pressure freezing followed by freeze-substitution (HPF/FS) results in excellent preservation of cell structure in these difficult-to-fix samples.
View Article and Find Full Text PDFThree-dimensional imaging of cells using electron tomography enables analysis of cell structure at unprecedented resolution. The preparation of cells for tomography using rapid freezing followed by freeze-substitution is an essential first step to ensure the optimal preservation of the cell structure for 3D studies. This protocol outlines a method for obtaining well-preserved cells using high-pressure freezing followed by freeze-substitution.
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