Publications by authors named "T Garate"
Cysticerci of Taenia solium cause cysticercosis, with neurocysticercosis (NCC) as the major pathology. Sensible and specific recombinant antigens would be an source of antigen for immunodiagnosis. The objective of this work was the molecular characterization and evaluation, of three news recombinant antigens (TsF78, TsP43 and TsC28), obtained by screening of a Taenia solium cDNA library.
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- The enzyme-linked immunoelectrotransfer blot (EITB) assay is a promising method for diagnosing neurocysticercosis (NCC) by detecting specific antibodies in serum.
- Presence of T. solium antigens is indicated by glycoprotein bands, and their patterns may enhance diagnostic accuracy.
- The study found that more glycoprotein bands correlate with vesicular cysts and negatively with calcified cysts, indicating the tests can predict cyst stage and location effectively.
Article Synopsis
- The study evaluated the effectiveness of seven immunodiagnostic tests for neurocysticercosis (NCC) using serum samples from NCC patients, other neurological patients, and healthy controls.
- The enzyme-linked immunosorbent assay (ELISA) for Taenia solium antibody demonstrated the highest sensitivity at 81%, making it a better option than other tests.
- Overall specificity was high across tests, but the HP10 antigen detection was notably tied to the presence of active parasites, suggesting it could be beneficial in diagnosing NCC, particularly in low-resource settings.
Recombinant proteins expressed in E. coli are frequently purified by immobilized metal affinity chromatography (IMAC). By means of this technique, tagged proteins containing a polyhistidine sequence can be obtained up to 95% pure in a single step, but some host proteins also bind with great affinity to metal ions and contaminate the sample.
View Article and Find Full Text PDFBackground: Onchocerciasis, also known as river blindness, is a parasitic disease. More than 99 percent of all cases occur in Africa. Bioko Island (Equatorial Guinea) is the only island endemic for onchocerciasis in the world.
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