[Characterization of enzymatic activity and structure of vesicles of human erythrocyte membranes].

Enzymatic activity and structural peculiarities of human erythrocyte membranes obtained by different methods, are analyzed in the paper. Our studies have demonstrated different enzymatic activity of the preparations of right side- and inside-out vesicles of human erythrocyte membranes. Fluorescence probes of pyrene and ANS have shown that these membrane preparations have different physical characteristics of lipid and conformational properties of membrane proteins and differ in the composition of their protein-lipid ensembles.

[Cu(2+) complexes as inhibitors of free radical oxidation of lipids].

Cu(2+) complexes of chelate ligands have been studied for their effect on oxidation of rat blood plasma lipids, sphingomyelin, lecithin by the method of chemoluminescence. The studied Cu(2+) complexes have been shown to possess pro-oxidant and antioxidant properties depending on their concentration. Antioxidant activity of alpha-tocopherol, ionol (2,6-di(tert)-butyl-4-methylphenol) and Cu(2+) complexes by the lipid oxidation has been compared.

[Correction of disorders of phosphorus metabolism after operations on the abdominal organs].

Hypophosphatemia and decreased content of ATP, 2,3-DPG in erythrocytes were found in 147 patients operated upon organs of the abdominal cavity. Hypophosphatemia was more pronounced after operations for ulcer disease of the stomach and duodenum, acute pancreatitis and commissural ileus. An intravenous injection of potassium dihydrogen phosphate++ prevents the development of hypophosphatemia and concomitant decrease of the content of nonorganic phosphorus, ATP, 2,3-DPG in erythrocytes and may improve the postoperative period in these patients.

[Disordered phosphorus metabolism and its correction in the acute period of severe craniocerebral trauma].

Marked hypophosphatemia was discovered in 78 patients in the acute period of severe craniocerebral injury. It led to a decrease of the level of 2,3-diphosphoglycerate (2,3 DPG) and ATP in the red cells. The reduced ATP level in the red cells was one of the causes of reduced Ca2+ ATPase activity in their membranes.

[The pharmacological correction of disorders of the oxygen transport function of the blood in peritonitis patients].

Arterial hypoxemia, decrease in non-organic phosphorus, 2,3-DPG, ATP erythrocyte levels and associated deterioration of O2 tissue supply have been observed in 106 patients with peritonitis. Intravenous administration of propranolol, potassium dihydrophosphate and inosine prevented a decrease in erythrocyte phosphate level, improved oxygen and energy tissue supply and reduced the duration of the patient stay in hospital.