The analysis was applied to serum and supernatants of day cultures of blood cells of patients with autoimmune thyroiditis and healthy women to determine the content of cytokins (IFN-gamma, TNF-alpha, IL-beta, IL-6, IL-10, L-17) and lactoferrin. The test-system "Cytokin-Stimul-Best" was applied. It is established that in patients with autoimmune thyroiditis the proliferative response of cells to mitogen stimulation is lowered in vitro: the levels of alpha-tumor necrosis factor interleikin-6, lactoferrin.
View Article and Find Full Text PDFThe standardized technique to detect the blood cells cytokine production ex vivo was developed. The complex of mitogens of phytohemagglutinin P, phytohemagglutinin M, lipopolysaccharide, concanavalin A is applied to provide the mitogen activation. This approach permits to activate the different types of immunocompetent cells and to induce the secretion of cytokine representative series.
View Article and Find Full Text PDFThe authors examined serum in patients with ovarian cancer (OC; a disseminated process), ovarian cystadenoma (OCA), or external endometriosis (EM) before treatment and in apparently healthy females (a control) for the content of some acute-phase proteins and cytokines to clarify the specific features of changes in their concentrations in relation to the type of the proliferative process. It was shown that in OC, there were significant reductions in the levels of alpha2-macroglobulin (MG), plasmin (PL), alpha1-antitrypsin (AT) and statistically significantly increases in the content of lactoferrin (LF), interleukin (IL)-6, IL-8, Ig, and the regulatory transport complex of P--M. In M, the concentrations of AT were lower and those of IL-6, IL-8, and PL-MG were higher (to a lesser degree than those in OC).
View Article and Find Full Text PDFThe study was undertaken to search for additional diagnostic criteria allowing the depth of myocardial damage to be estimated in males aged 57.2 +/- 9.6 years.
View Article and Find Full Text PDFThe morphology and virus localization were studied in the microcapsulated measles vaccine formulation involving polyacrylic acid (PAA) copolymers as a matrix. Transmission electron microscopy and phosphotungsic staining at a pH value of 2 to 7 showed that the morphology of microparticles was related to the value of pH and to the concentration of a polymer in the matrix. In the neutral medium, the microcapsules had the sizes of 0.
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