Estimated stearoyl-CoA desaturase activity mediates the associations of total cysteine with adiposity: The Maastricht Study.

Background: Plasma sulfur amino acids (SAAs), particularly cysteine, are associated with obesity. One proposed mechanism is the altered regulation of the stearoyl-CoA desaturase (SCD) enzyme. Changes in the SCD enzyme activity have been linked to obesity, as well as to plasma SAA concentrations.

Scalable co-sequencing of RNA and DNA from individual nuclei.

The ideal technology for directly investigating the relationship between genotype and phenotype would analyze both RNA and DNA genome-wide and with single-cell resolution; however, existing tools lack the throughput required for comprehensive analysis of complex tumors and tissues. We introduce a highly scalable method for jointly profiling DNA and expression following nucleosome depletion (DEFND-seq). In DEFND-seq, nuclei are nucleosome-depleted, tagmented and separated into individual droplets for messenger RNA and genomic DNA barcoding.

Outcomes of Zero Power Intraocular Lenses: Insights into the Keratometric Index and Axial Length.

Purpose: We analyzed intraocular lens (IOL) power calculation in a series of patients with a zero diopter power implant. The idea was to bypass the Estimated Lens Plane (ELP), an important variable in normal IOL calculation.

Setting: Large multi-center group practice.

Effect of Hubbard U-corrections on the electronic and magnetic properties of 2D materials: a high-throughput study.

We conduct a systematic investigation of the role of Hubbard U corrections in electronic structure calculations of two-dimensional (2D) materials containing 3 transition metals. Specifically, we use density functional theory (DFT) with the PBE and PBE+U approximations to calculate the crystal structure, band gaps, and magnetic parameters of 638 monolayers. Based on a comprehensive comparison to experiments we first establish that the inclusion of the U correction worsens the accuracy for the lattice constants.

Hypoxia-inducible factor 2α promotes protective Th2 cell responses during intestinal helminth infection.

Th2 cells must sense and adapt to the tissue milieu in order to provide protective host immunity and tissue repair. Here, we examined the mechanisms promoting Th2 cell differentiation and function within the small intestinal lamina propria. Single cell RNA-seq analyses of CD4 T cells from the small intestinal lamina propria of helminth infected mice revealed high expression of the gene , encoding the transcription factor hypoxia-inducible factor 2a (HIF2α).