Induction of emesis with apomorphine using a novel gingival administration method in dogs.

Objective: To describe a novel method of inducing emesis in the dog using gingival administration of apomorphine, compare the efficacy of inducing emesis with gingival apomorphine to conjunctival apomorphine, and describe adverse effects associated with the gingival route.

Design: Retrospective study from January 2017 to September 2018.

Setting: Independent all-hours primary and secondary emergency and critical care referral center.

Cultured human keratinocytes for optical transmission measurement.

The challenges of measuring optical properties of human tissues include the thickness of the sample, homogenization, or crystallization from freezing of the tissue. This investigation demonstrates a method to avoid these problems by growing optically thin samples of human keratinocytes as a substitute for ex vivo epidermis samples. Several methods of growth were investigated.

Comparison of 1540-nm laser-induced injuries in ex vivo and in vitro rabbit corneal models.

Despite the increasing use of infrared lasers in medical, industrial, and military settings, data on threshold radiant exposures and median effective dose (ED(50)) as they relate to laser-tissue interaction are limited. Our goals were to determine the ED(50) for single-pulse, 1540-nm laser exposures in ex vivo and in vitro rabbit corneal models and to characterize the histopathological changes associated with the laser-tissue interaction. An erbium-glass laser was used to deliver single, 1540-nm wavelength pulses to 27 ex vivo and 24 in vitro rabbit corneal models.

Alginate as a new biomaterial for the growth of porcine retinal pigment epithelium.

Objective: Determine the effect of a 3-dimensional alginate matrix on the growth and differentiation of cells isolated from porcine retinal pigment epithelium (RPE).

Procedures: Porcine RPE cells were harvested from enucleated eyecups, isolated by differential gravity sedimentation and cultured in either alginate alone (Group 1) or on plastic tissue culture plates followed by alginate (Group 2). Group 1 cells were cultured in alginate to evaluate the efficacy of the matrix as a culture medium.

Proliferation of canine intervertebral disk chondrocytes in three-dimensional alginate microsphere culture.

Proliferation of chondrocytes from nucleus pulposus (NP) and anulus fibrosus (AF) was confirmed in three-dimensional culture using alginate microspheres. Cells isolated from NP and AF were incorporated in microspheres and cultured for 14 days. Round mononuclear cells of 20-25 microm in diameter proliferated and formed aggregates.