[Isolation of the Chikungunya virus in Moscow from the Indonesian visitor (September, 2013)].

The results of the virological identification of the Chikungunya fever case in Moscow (September, 2013) in an Indonesian visitor are presented. The clinic, electron microscopy, and molecular genetic data are discussed. The Ghikungunya virus (CHIKV) strain CHIKVILEIV-Moscow/1/2013 belonging to the Asian genotype (ID GenBank KF872195) was deposited into the Russian State Collection of viruses (GKV 1239; 18.

[The mode of spot test of plague and pseudotuberculosis agents mix cultures].

It is supposed to implement the polymerase chain reaction with mix of two pairs of "chromosome" primers "vlm12for'/"IS216rev" and "JS for"/JSrev" species-specific for Y. pestis and Y. psdtbc correspondingly in spot test of plague and pseudotuberculosis.

[Comparison of efficacy of tests for differentiation of typical and atypical strains of Yersinia pestis and Yersinia pseudotuberculosis].

Aim: To characterize species specificity of officially recommended tests for differentiation of Yersiniapestis and Yersinia pseudotuberculosis and propose additional tests allowing for more accurate identification.

Materials And Methods: Natural, laboratory and typical strains oftwo Yersinia species were studied using microbiological, molecular and biochemical methods. For PCR species-specific primers complementary to certain fragments of chromosomal DNA of each species as well as to several plasmid genes of Y.