[Comparison of leukocyte filters for erythrocyte concentration].

Unlabelled: We compared the following leukocyte filter blood systems (BBS): Biotrans Bio R01 Plus, Diamed Sepacell RS-200, Pall BPF4 (log 4 filters) and Biotrans Bio R01 (log 3 filter). We evaluated the leukocyte removal, handling, the increase of free hemoglobin and the loss of red blood cells (RBC) by filtration. A total of 54 buffy-coat-reduced RBCs were suspended in 200 ml PAGGS-M, stored at +4 degrees C for 24 h and filtered subsequently.

Detection of HLA antibodies by an enzyme-linked photometric immune phagocytosis inhibition test.

The immune phagocytosis inhibition test (IPI) has been described as a sensitive method for the detection of cytotoxic and non-cytotoxic HLA antibodies. We performed a photometric IPI and compared this technique with the conventional microscopic IPI. In further investigations we used the photometric IPI in comparison with the lymphocytotoxicity test (LCT) for the detection of HLA antibodies.

[Oxygen affinity and regeneration capacity of SAG-M and PAGGS-M stored erythrocytes].

In order to estimate the leftward shift of the oxygen dissociation curve of hemoglobin following storage of red blood cells (rbc) in the additive solutions SAG-M and PAGGS-M, respectively, we performed blood gas analyses after equilibrating the cells with a gas mixture containing 4% of O2 and 5% of CO2 at pH 7.4 and 37 degrees C. Additionally, we took advantage of these nearly physiological conditions to measure the recovery of the hemoglobin function in vitro.

[Comparison of erythrocyte concentrations of ATP, ADP, AMP and 2,3-BPG in storage of five different additive solutions and in serum].

In this study, we compared the ability of serum and of 5 additive solutions for the liquid storage of red blood cells to preserve the cellular contents of 2,3-BPG and the adenylate nucleotides ATP, ADP and AMP. As to the adenylates, PAGGS-M showed the best results. However, the difference to SAG-M was significant only after 7 weeks.