[Evaluation of Indicators of Postejaculation Maturation of Spermatozoa of Bos taurus Using a Chlortetracycline Test].

Postejaculation maturation of spermatozoa (capacitation and acrosomal exocytosis) in bovine sperm was assessed using the method of staining with chlortetracycline and inhibitor analysis to identify the transition ways of calcium of intracellular depots under the influence of highly dispersed silica. It was shown that highly dispersed silica in a concentration of 0.001% stimulates capacitation but has no impact on the acrosome exocytosis in bovine sperm.

[Effect of vitrification on the functioning of the structural elements of the cytoskeleton in porcine oocytes].

On the basis of inhibition analysis using inhibitors of protein kinase A and polymerization of microfilament under the influence oftheophylline, GDP and GTP have studied the release of Ca2+ from intracellular stores (ryanodin- and inositol-1 ,4,5-trisphosphate) of native and devitrified porcine oocytes. It is found that theophylline, as well as GDP stimulate the release of Ca2+ from intracellular stores in the native and devitrified oocytes, but a further release of Ca2+ from intracellular stores under the joint action of theophylline and GDP was observed only in native oocytes. Inhibitors of protein kinase A and polymerization of microfilaments in native oocytes have a negative impact on the further release of Ca2+ from intracellular stores under the joint action of theophylline and GDP.

[Clinical efficacy of functional nutrition in patients with gastrointestinal disorders].

Aim: To identify micronutrient deficiencies in patients with functional bowel diseases (FBD) and to reveal their correction with functional foods and probiotics.

Subjects And Methods: The health status was evaluated in 90 patients aged 18 to 67 years with FBD. All the patients were randomized into 3 groups according to the treatment regimen.

[Solved and unsolved problems of nutritional support in surgical gastroenterology].

The article has revealed the relevance of nutriciology as a science, classification and types of nutritional support. Nutritional support--diagnostics and the metabolic correction of arising violations of various kinds of homeostasis, aimed at ensuring the functioning of the systems of protein-energy synthesis and intermediate exchange of nutrients, vitamins, macro--and microelements, or a temporary replacement therapy of their failure. The notions of "lack of food", "artificial" food, protein-energy insufficiency with the exception of the syndromes of malabsorption, alimentary anemia, the effects of protein-energy malnutrition, consumptive disease, starvation have been interpreted.

[Identification of mechanisms of the calcium signaling at influence of estradiol on porcine oocytes, stimulated by theophillin and somatotropin].

Through the use of inhibitory analysis by using the fluorescent probe chlortetracycline examined the effects of estradiol on the mobilization of Ca2+ from intracellular stores of porcine oocytes stimulated by growth hormone and theophylline. It is shown that somatotropin or theophylline stimulates the mobilization of Ca2+ from intracellular stores of oocytes in control group, while their combined action does not lead to an additional exit of Ca2+ from intracellular stores. Inhibitor of protein kinase C had no effect on the release of Ca2+--from oocyte stimulated by growth hormone or theophylline.