Impact of Growth Conditions on Morphology Characterized by Atomic Force Microscopy.

This work is dedicated to the characterization by Atomic Force Microscopy (AFM) of , bacteria having high potential in biotechnology. They were first studied first in optimal conditions in terms of culture medium and temperature. AFM revealed a more-or-less elongated morphology with typical dimensions in the micrometer range, and an organization of the outer membrane characterized by the presence of long and randomly distributed ripples, which are likely related to the organization of lipopolysaccharides (LPS).

Characterization of cadmium accumulation and phytoextraction in three species of the genus Atriplex (canescens, halimus and nummularia) in the presence or absence of salt.

This study aims to establish for the first time a comparison between the resistance to cadmium (Cd) stress of three halophyte species, Atriplex canescens, Atriplex halimus and Atriplex nummularia in addition to their already known tolerance for salt and drought. Plants were exposed to CdCl (20 and 50 μM) in the presence or in the absence of salt (50 mM NaCl) for one and two months. The amount of accumulated Cd was determined in the roots and leaves as well as the amount excreted on the surface of the leaves.

Lipid monolayer as a simple model membrane for comparative assessment of the photodynamic therapy photosensitizer efficiency via macroscopic measurements.

Cellular membrane is one of the main targets of photodynamic therapy. Its high complexity has led to the study of the efficiency of photosensitizers on artificial lipid systems mimicking membranes. However, the preliminary analysis of this efficiency remains limited due to difficulty of the model construction and/or implementation of the required measurement techniques.

Detrimental impact of silica nanoparticles on the nanomechanical properties of Escherichia coli, studied by AFM.

Despite great innovative and technological promises, nanoparticles (NPs) can ultimately exert an antibacterial activity by affecting the cell envelope integrity. This envelope, by conferring the cell its rigidity and protection, is intimately related to the mechanical behavior of the bacterial surface. Depending on their size, surface chemistry, shape, NPs can induce damages to the cell morphology and structure among others, and are therefore expected to alter the overall mechanical properties of bacteria.

Hydrocalumite Thin Films for Polyphenol Biosensor Elaboration.

Hybrid thin films based on Hydrocalumite (CaAlCl layered double hydroxide LDH) and tyrosinaseenzyme have been used for the elaboration of a high sensitive amperometric biosensor detecting polyphenols extracted from green tea. Structural properties of LDH nanomaterials were characterized by X-ray powder diffraction and Infra-Red spectroscopy, confirming its crystalline phase and chemical composition. CaAlCl-LDHs-thin films were deposited by spin-coating, and studied by atomic force microscopy to obtain information about the surface morphology of this host matrix before and after enzyme's immobilization.