Extensive environmental survey of free-living amoebae and their elusive association with Mycobacterium bovis or Mycobacterium avium subsp. paratuberculosis.

Free-living amoebae (FLA) are described as environmental reservoirs for some bacteria able to resist their phagocytosis. In the environment, the fate of Mycobacterium bovis (Mbo) and Mycobacterium avium subsp. paratuberculosis (Map) responsible for bovine tuberculosis and paratuberculosis, respectively, remains poorly understood and is considered potentially problematic in the eradication and control of these diseases.

Complete genome sequence of an ovine ancestral strain of subspecies 6756.

The complete genome sequence of the most ancestral type SI strain of subspecies 6756, isolated from a sheep, was determined. The genome was sequenced using PacBio technology, yielding a genome size of 4,830,294 nucleotides with no identified plasmids.

Presence of Non-Tuberculous Mycobacteria Including subsp. Associated with Environmental Amoebae.

One of the obstacles to eradicating or Johne's Disease (JD) seems to be the persistence of subsp. () in the environment due to its ability to survive alone or vectorized. It has been shown that is widely distributed in soils and water.

Features of Complete Genomes Belonging to 5 Different Lineages.

Mammalian tuberculosis (TB) is a zoonotic disease mainly due to (). A current challenge for its eradication is understanding its transmission within multi-host systems. Improvements in long-read sequencing technologies have made it possible to obtain complete bacterial genomes that provide a comprehensive view of species-specific genomic features.