How ready is speech-to-text for psychological language research? Evaluating the validity of AI-generated English transcripts for analyzing free-spoken responses in younger and older adults.

For the longest time, the gold standard in preparing spoken language corpora for text analysis in psychology was using human transcription. However, such standard comes at extensive cost, and creates barriers to quantitative spoken language analysis that recent advances in speech-to-text technology could address. The current study quantifies the accuracy of AI-generated transcripts compared to human-corrected transcripts across younger (n = 100) and older (n = 92) adults and two spoken language tasks.

Why replication is important in landscape genetics: American black bear in the Rocky Mountains.

We investigated how landscape features influence gene flow of black bears by testing the relative support for 36 alternative landscape resistance hypotheses, including isolation by distance (IBD) in each of 12 study areas in the north central U.S. Rocky Mountains.

Cloning, isolation and characterization of human tumor in situ monoclonal antibodies.

A bacterially expressed human antibody (Ab) library (diversity approximately 10(5)) was generated from tumor-infiltrating B lymphocytes present in tissue isolated from a colon tumor. Immunoglobulin (IgG) heavy and light chain variable regions were amplified without isolating or enriching B cells, cloned into a phage-expression vector, and soluble antigen-binding fragment (Fabs) from >10(5) members of the library were screened rapidly by two distinct and complementary methodologies. In the first approach, soluble Fabs were screened by enzyme-linked immunosorbent assay (ELISA) on tumor cell monolayers.

Use of a peptide mimotope to guide the humanization of MRK-16, an anti-P-glycoprotein monoclonal antibody.

A mimotope-guided strategy for engineering antibodies directed against orphan targets or antigens that are difficult to purify was developed and used to humanize the murine MRK-16 monoclonal antibody (mAb). MRK-16 recognizes a conformational epitope of a 170-kDa membrane protein, termed P-glycoprotein (P-gp). Elevated expression of P-gp on tumor cells is associated with resistance to cytotoxic drugs, a major obstacle in chemotherapy.

Dietary supplementation with gamma-linolenic acid alters fatty acid content and eicosanoid production in healthy humans.

To understand the in vivo metabolism of dietary gamma-linolenic acid (GLA), we supplemented the diets of 29 volunteers with GLA in doses of 1.5-6.0 g/d.