Cannabis cultivation and processing is becoming an important industry in the United States and Canada. The industry employs over 400,000 workers in the United States and is growing rapidly. Both natural sunlight and artificial lamp-generated radiation are commonly used to grow cannabis plants.
View Article and Find Full Text PDFThe subventricular zone (SVZ) of the lateral ventricles represents a main region where neural stem cells (NSCs) of the mature central nervous system (CNS) reside. Bone Morphogenetic Proteins (BMPs) are the largest subclass of the transforming growth factor-β (TGF-β) superfamily of ligands. BMP4 is one such member and plays important roles in adult NSC differentiation.
View Article and Find Full Text PDFThis protocol entails a simple method for isolation, culturing, and differentiation of adult neural stem cells from the dentate gyrus in the hippocampus and the subventricular zone of adult mice. Cultured adult neural stem cells are an important model to investigate stem cell properties such as proliferation and differentiation and to expand the understanding of plasticity in the adult brain. For complete details on the use and execution of this protocol, please refer to Isaksen et al.
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