Publications by authors named "T Buchou"
Article Synopsis
- The histone variant H3.3, produced by two genes (H3f3a and H3f3b) with the same amino-acid sequence, is crucial for spermatogenesis, although its specific role was unclear.
- Research using genetically modified mice revealed that only H3.3B (not H3.3A) is essential for male fertility, affecting the transition during meiosis.
- Further analysis indicated that the absence of H3.3B causes changes in gene expression, notably increasing sex chromosome activity and altering piRNA cluster expression, highlighting H3.3B's significant regulatory role in spermatogenesis.
The discoid shape of resting platelets is maintained by a peripheral, circular bundle of microtubules called marginal band. Marginal band microtubules are acetylated on lysine 40 of the alpha-tubulin subunits. We have previously shown that the deacetylase HDAC6 is responsible for tubulin deacetylation in platelets and that the hyperacetylated state of the microtubules in platelets correlates with faster activation/spreading kinetics, pointing to a regulatory role of this modification.
View Article and Find Full Text PDFThe molecular basis of residual histone retention after the nearly genome-wide histone-to-protamine replacement during late spermatogenesis is a critical and open question. Our previous investigations showed that in postmeiotic male germ cells, the genome-scale incorporation of histone variants TH2B-H2A.L.
View Article and Find Full Text PDFBackground: Altering the biochemical makeup of chromatin by the incorporation of histone variants during development represents a key mechanism in regulating gene expression. The histone variant H2A.B, H2A.
View Article and Find Full Text PDFArticle Synopsis
- Nut is an oncogenic protein associated with NUT midline carcinoma, but its role in male germ cell development has been poorly understood.
- The study finds that Nut is expressed in post-meiotic spermatogenic cells and is vital for histone H4 hyperacetylation, which is crucial for the transition from histones to protamines during sperm development.
- Inactivating Nut leads to male sterility by halting spermatogenesis at the histone removal stage, revealing its critical role in genome compaction in male germ cells.