Human induced pluripotent stem cells (BIONi010-C) generate tight cell monolayers with blood-brain barrier traits and functional expression of large neutral amino acid transporter 1 (SLC7A5).

The barrier properties of the brain capillary endothelium, the blood-brain barrier (BBB) restricts uptake of most small and all large molecule drug compounds to the CNS. There is a need for predictive human in vitro models of the BBB to enable studies of brain drug delivery. Here, we investigated whether human induced pluripotent stem cell (hiPSC) line (BIONi010-C) could be differentiated to brain capillary endothelial- like cells (BCEC) and evaluated their potential use in drug delivery studies.

Clinical correlates of resectability and survival in gastric carcinoma.

The course of 201 patients with carcinoma of the stomach treated from 1962 through 1966 was followed with 97% determinacy for 10 years. The actual five year survival rate was 11%; the ten year rate was 7%. The mean duration of survival was 5.

Humoral stimulation of cell proliferation in small bowel after transection and resection in rats.

Possible humoral involvement in the control of postresectional intestinal adaptation was tested in individual rats and in pairs of rats connected in vascular parabiosis for 48 hr. In individual rats, transection of the jejunum produced transient mucosal hyperplasia in the mid and distal small intestine, independent of the effect of laparotomy and of handling the bowel. Jejunal resection caused more intense and sustained distal hyperplasia, with increases in midbowel RNA content (38%), DNA content (16%), and DNA specific activity (68%) over values 48 hr after transection.

Onset of cell proliferation in the shortened gut: growth after subtotal colectomy.

Cell proliferation and remodeling of the small intestine were studied after subtotal colectomy in the rat. After an initial fall in nucleic acid contents, there was a trend to increasing amounts of RNA throughout the intestine on the fourteenth postoperative day; by the thirtieth day RNA and DNA contents were increased about 20 percent. DNA synthesis increased after 5 days.

Femorofemoral bypass graft in intra-aortic balloon counterpulsation.

Femorofemoral arterial crossover grafts were used in three patients to avoid or correct limb ischemia during intra-aortic balloon counterpulsation. In retrospec, these grafts should have been used in two earlier patients in whom the balloon was removed prematurely. They are applied to the femoral artery just distal to the site of insertion of the balloon or the the balloon access graft.