Publications by authors named "T Bott"
A simple method for testing and controlling inhibition in soil and sediment samples for qPCR.
J Microbiol Methods
September 2023
The presence of polymerase-chain-reaction (PCR) inhibitors in many environmental samples can make reliable and repeatable quantitative-polymerase-chain-reaction (qPCR) analysis difficult without sample dilution. To estimate an optimal sample dilution for qPCR and reduce effects of inhibition, a simple test based on multiple dilution series of samples is presented that avoids the use of internal controls and standards reducing complexity and cost.
View Article and Find Full Text PDFArticle Synopsis
- Grasslands cover about one-third of the land on Earth and are important for storing soil organic carbon, which is essential for ecosystem services.
- Most research on soil carbon has focused on croplands, but with a goal of achieving "net zero" emissions by 2050, grasslands are being explored for their carbon storage potential, particularly through the use of biochar.
- The study evaluates the challenges and benefits of using biochar in different types of grasslands, highlighting the need for further research to ensure that increasing carbon storage does not compromise other ecosystem services.
Dispiropiperazine compounds are a class of molecules known to confer biological activity, but those that have been studied as cell cycle regulators are few in number. Here, we report the characterization and synthesis of two dispiropiperazine derivatives: the previously synthesized spiro[2',3]-bis(acenaphthene-1'-one)perhydrodipyrrolo-[1,2-a:1,2-d]-pyrazine (SPOPP-3, 1), and its previously undescribed isomer, spiro[2',5']-bis(acenaphthene-1'-one)perhydrodipyrrolo-[1,2-a:1,2-d]-pyrazine (SPOPP-5, 2). SPOPP-3 (1), but not SPOPP-5 (2), was shown to have anti-proliferative activity against a panel of 18 human cancer cell lines with IC values ranging from 0.
View Article and Find Full Text PDFArticle Synopsis
- * Replanted digits showed 58% of the range of motion of uninjured digits, while both groups had similar grip strength and sensory recovery measurements.
- * Patients who underwent replantation experienced significantly less pain at rest and during activity compared to those who had an amputation.
Secondary lymphoedema is a common concern for people with cancer. , Clinical Trials Database Nurse, Cancer Research UK (Trevor.Bott@cancer.
View Article and Find Full Text PDF