Functional state of sportsmen with mitral valve prolapse.

The study covered functional peculiarities in 541 professional sportsmen. Mitral valve prolapse (in 132 examinees) appeared to be associated with reliably lower levels of reticulocytes, leucocytes, lymphocytes CD4+, immunoglobulines G, magnesium, and higher cortisol level, in comparison with the sportsmen having no mitral valve prolapse. The athletes with mitral valve prolapse demonstrated more frequent changes in ECG, reliably lower performance in treadmill test, slower recovery of heart rate after exertion is over.

Complementary directed modifications of nucleic acids and oncogene-directed mutagenesis in vivo.

High reactivity of the polyalkylating ss oligomers that were sense or antisense 30-200-mers containing sequences complementary to E1 oncogenes of simian adenovirus SA7 and one alkylating residue -CH2CH2N(C2H5OH) (CH2)3N(Ph-p-CH2OH)CH2CH2Cl per each 25 bases of oligomers was demonstrated in vitro by alkylation of ss DNA of recombinant M 13 mp8E1 and mp9E1 phages with inserted E1 sequences of adenovirus oncogene and then by followed complete and selective elimination of E1 sequences from recombinant ss DNA. Treatment of rodent cell cultures transformed by oncogenic SA7 with polyalkylating oligomers which are complementary to the long region of the minus or plus chains of E1 DNA of SA7 revealed a rather high extent of mutant cell clones formation. The cells formed were normalized; they had lost some properties of the transformed cells.