Protocol for CRISPR-Cas9-mediated induction of KMT2A rearrangements in cell line and umbilical cord blood hematopoietic stem and progenitor cells.

KMT2A rearrangements are associated with a poor clinical outcome in infant, pediatric, and adult acute lymphoblastic and myeloid leukemia. Here, we present a protocol to reconstruct chromosomal translocations with different partner genes of KMT2A in vitro. We describe steps for patient-specific single guide RNA (sgRNA) design, optimized sgRNA in vitro transcription, detailed purification of hematopoietic stem and progenitor cells (HSPCs) from umbilical cord blood (UCB), and CRISPR-Cas9 editing of the test cell line K562 as well as UCB HSPCs.

Relationship between subjectively-rated and objectively-tested physical function across six different medical diagnoses.

Objective: To quantify and compare associations and relationships between self-rated and tested assessments of mainly mobility-related physical function in different diagnoses.

Design: Six longitudinal cohort studies before and after inpatient rehabilitation.

Patients: Patients with whiplash-associated disorder (n = 71), low back pain (n = 121), fibromyalgia (n = 84), lipoedema (n = 27), lymphoedema (n = 78), and post-acute coronary syndrome (n = 64).

[Validation and reliability testing of the "Athlete Fear Avoidance Questionnaire (AFAQ)"].

Background: Sports injuries may have severe implications for an athlete's career. Psychological factors play an important role on an athlete's journey from the injury to the return to competitive sports. These factors are often insufficiently evaluated, which may lead to prolonged downtime.

Tumor-derived GDF-15 blocks LFA-1 dependent T cell recruitment and suppresses responses to anti-PD-1 treatment.

Immune checkpoint blockade therapy is beneficial and even curative for some cancer patients. However, the majority don't respond to immune therapy. Across different tumor types, pre-existing T cell infiltrates predict response to checkpoint-based immunotherapy.