Are the evaluation criteria used in preclinical endodontic training courses relevant? A preliminary study.

This study aimed to evaluate the relevance of a conventional evaluation protocol (CEP) for preclinical endodontic training. Seven dental students performed root canal treatments on an extracted human molar which was then evaluated by CEP (clinical and radiographic observations, including preparing the access cavities, preparing and filling the root canal, and detecting iatrogenic errors). A reference evaluation protocol (REP) based on micro-computed tomographic images analysis was used as a control.

Well-organized spheroids as a new platform to examine cell interaction and behaviour during organ development.

We present an experimental method allowing the production of three-dimensional organ-like structures, namely microtissues (MTs), in vitro without the need for exogenous extracellular matrix (ECM) or growth factors. Submandibular salivary glands (embryonic day ED14), kidneys (ED13) and lungs (ED13) were harvested from mouse embryos and dissociated into single cells by enzyme treatment. Single cells were seeded into special hanging drop culture plates (InSphero) and cultured for up to 14 days to obtain MTs.

A novel anatomical ceramic root canal simulator for endodontic training.

Introduction: Endodontic therapy is often complicated and technically demanding. The aim of this study was to develop a reproducible biomimetic root canal model for pre-clinical and postgraduate endodontic training.

Material And Methods: A specific ceramic shaping technique (3D printing and slip casting of a root canal mould) was developed to reproduce canal systems with the desired shape and complexity using a microporous hydroxyapatite (HAp)-based matrix.

Three-dimensional Micro-culture System for Tooth Tissue Engineering.

The arrangement of cells within a tissue plays an essential role in organogenesis, including tooth development. Progress is being made to regenerate teeth by reassociating dissociated embryonic dental cells and implanting them in vivo. In the present study, we tested the hanging drop method to study mixed epithelial-mesenchymal cell reorganization in a liquid instead of semisolid medium to see whether it could lead to tooth histogenesis and organogenesis.

Immunomodulation stimulates the innervation of engineered tooth organ.

The sensory innervation of the dental mesenchyme is essential for tooth function and protection. Sensory innervation of the dental pulp is mediated by axons originating from the trigeminal ganglia and is strictly regulated in time. Teeth can develop from cultured re-associations between dissociated dental epithelial and mesenchymal cells from Embryonic Day 14 mouse molars, after implantation under the skin of adult ICR mice.