[Neutralizing antibodies to human gamma-interferon].

Spleen lymphocytes from normal subjects who suddenly died were used as cells producing gamma-interferon. One spleen yielded (3-8) X 10(8) viable cells which made it possible to prepare from 3 to 81 of splenocyte suspension culture. Staphylococcal enterotoxin A (SEA) and B (SEB) were used as gamma-interferon inducers.

[Simple method of determining the antiproliferative activity of human interferons].

A simple method for quantitative estimation of the antiproliferative activity of human interferon is described. The method is based on direct visual estimation (with the use of an inverted microscope) of various doses of interferon with respect to proliferation and colony formation of human transplantable tumor HeLa cells grown in Nells of plastic plates for microcultures. Single HeLa cells (target cells) are plated out on a monolayer of human embryo fibroblasts (HEF), which morphologically differ from the target cells (HeLa) and play the role of feeders.

[Effect of T-activin on the production of immune interferon].

A study was made of the effect of T-activin on the biosynthesis of immune gamma-interferon. It was shown that in 27% of patients with chronic nonspecific pulmonary diseases, production of gamma-interferon by lymphocytes was substantially reduced during exacerbation of inflammatory process in the lungs. It was discovered that T-activin was not an interferon inductor but enhanced its synthesis in patients with a low capacity of producing immune interferon even at small doses of interferon inductor.