[GnRH analogues containing SV-40 virus T-antigen nuclear localization sequence].

To improve the efficiency of anticancer drugs due to their delivery to intracellular targets a set of GnRH analogues containing nuclear localization signal (NLS) of SV-40 virus large T-antigen have been synthesized. NLS was attached to the parent molecule via ε-amino group of D-Lysine in position 1 or 6 of peptide sequence using orthogonal protection strategy. The biological activity studies revealed that incorporation of NLS moiety significantly increases cytotoxic activity of palmitoyl-containing GnRH analogues in vitro.

[Luliberin analogues exhibiting a cytotoxic effect on tumor cells in vitro].

Luliberin analogues modified at the N-terminus were synthesized to search for drugs exerting a cytotoxic effect on cells of hormone-dependent tumors. A synthetic scheme effective in the preparation of analogues containing fatty acid residues was proposed. The cytotoxic effect of the peptides was studied on a number of cell lines of human tumors in vitro.

[Comparative evaluation of the effectiveness of vaccination of newborn infants with BCG and BCG-M vaccines].

A comparative effectiveness of the newborn BCG- and BCG-M vaccination was analysed as to the indices of primary infection, morbidity and its structure. The rate of postvaccination complications in 1,645,360 children, aged under 3 years, and living in the areas with different epidemiological situations for tuberculosis was studied too. 2,142,292 and 799486 healthy newborns were BCG- and BCG-M-vaccinated, respectively.

[Method for immunization against tuberculosis using BCG-M vaccine--a preparation with a reduced antigenic load].

Experimental and clinical investigations on the possibility of reducing the antigenic content of the vaccine intended for the immunization of newborn infants have been made. Experiments on guinea pigs and white mice have demonstrated that the reduced dose of the preparation, twice as low by weight (0.025 mg), produces the same level of protection against tuberculosis as the full dose (0.