Anesteziol Reanimatol
June 2003
Transfusion of blood components is not a method of choice at anemia, at the same time, hemotransfusion turns out often vitally indispensable at a pronounced reduction of hemoglobin especially in newborns. Indications for the transfusion of blood components demand that many factors of individual approach should be taken into consideration. Phenotyping of the blood of donor and of patient by using the standard serum methods and gel technology make it possible to avoid transfusion complications.
View Article and Find Full Text PDFThe paper discusses the problems of purification of Provachek's rickettsias used to prepare typhus vaccines and diagnostic kits. The currently available agents contain many admixtures of rickettsias products, more commonly yolk and chick embryo yolk sack cell detritus, which make it impossible to determine the true antigenic properties of rickettsias in the context of protection and diagnosis. By applying the well-known principle of distribution of biological particles in the two-phase systems of water soluble polymers, investigations were made to examine the distribution of rickettsias and their infected biological mass in the system: polyethylene glycol-6600, sodium-500 dextran sulfate, potassium phosphate, sodium chloride in order to prepare agents of pure rickettsias.
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