Multiple pathways from three types of sugar receptor sites to metabotropic transduction pathways of the blowfly: study by the whole cell-clamp experiments.

Multiple pathways from three types of multiple receptor sites to three types of metabotropic signal transduction pathways were investigated in the whole cell-clamp experiments using isolated labellar sugar receptor neurons (cells) of the adult blowfly, Phormia regina. First, the concentration-response curves of three types of sweet taste components specialized to multiple receptor sites were obtained: sucrose for the pyranose sites (P-sites), fructose for the furanose sites (F-sites), and l-valine for the alkyl sites (R-sites). Next, the effects of inhibitors such as 2', 5'-dideoxyadenosine on adenylyl cyclase in the cAMP pathway, LY 83583 on guanylyl cyclase in the cGMP pathway, and U-73122 on phospholipase C in the IP₃ pathway were examined.

[Fundamental study of the fat-suppressed three-dimensional coherent oscillatory state acquisition for the manipulation of image contrast (3D-COSMIC) sequence in the knee joint cartilage].

Fat-suppressed three-dimensional coherent oscillatory state acquisition for the manipulation of image contrast (3D-COSMIC) is a sequence that is based on fast imaging employing steady state acquisition (FIESTA) of balanced steady-state free precession (balanced SSFP). Since the data acquisition of steady-state transition is filled up with the center of k-space, improvement in the contrast of the cartilage, which is a low T₂/T₁ value domain, is expected. This time we report on the usability in applying the above sequence to cartilage imaging of the knee joint and comparing and examining this sequence with the sequence in the past from the viewpoints of the contrast and scan time.

Transduction pathways mediated by second messengers including cAMP in the sugar receptor cell of the blow fly: study by the whole cell clamp method.

The whole cell clamp method was directly applied to the sensory receptor neurons isolated from the adult labellar hair of the blow fly Phormia regina to locate the signal transduction pathways mediated by second messengers. First, the cAMP-mediated transduction pathway was examined to specify its location in the sugar receptor cell. Sugar receptor cell was identified by recording inward current flow under the voltage clamp applying sucrose solution to the surface of the taste neurons.