Cutting-edge tools for unveiling the dynamics of plasmid-host interactions.

The plasmid-mediated transfer of antibiotic resistance genes (ARGs) in complex microbiomes presents a significant global health challenge. This review examines recent technological advancements that have enabled us to move beyond the limitations of culture-dependent detection of conjugation and have enhanced our ability to track and understand the movement of ARGs in real-world scenarios. We critically assess the applications of single-cell sequencing, fluorescence-based techniques and advanced high-throughput chromatin conformation capture (Hi-C) approaches in elucidating plasmid-host interactions at unprecedented resolution.

A framework towards implementation of sequencing for antimicrobial-resistant and other health-care-associated pathogens.

Antimicrobial resistance continues to be a growing threat globally, specifically in health-care settings in which antimicrobial-resistant pathogens cause a substantial proportion of health-care-associated infections (HAIs). Next-generation sequencing (NGS) and the analysis of the data produced therein (ie, bioinformatics) represent an opportunity to enhance our capacity to address these threats. The 3rd Geneva Infection Prevention and Control Think Tank brought together experts to identify gaps, propose solutions, and set priorities for the use of NGS for HAIs and antimicrobial-resistant pathogens.

Unveiling complexities: Examining the role of traumatic loss in shaping the interplay between black maternal mental health and maternal bonding.

Black women are more likely to experience traumatic birthing events, more likely to experience perinatal depression, and less likely to receive mental health treatment than women of other racial and ethnic backgrounds, and yet largely overlooked in perinatal mental health research. This pilot study seeks to understand how unacceptable racial disparities and adverse perinatal outcomes influence Black maternal depression and maternal bonding by exploring how prior traumatic loss moderates the relationship between depression and bonding during a subsequent pregnancy among a sample of Black mothers. We use survey data collected from 75 Black mothers as part of the Black Fathers, Equal Partners in Promoting Maternal and Infant Health study, a collaboration between the University of Wisconsin Madison and the African American Breastfeeding Network in Milwaukee, Wisconsin, USA.

Incidental findings from cone-beam computed tomography in children and adolescents: a systematic review.

Purpose: The use of cone-beam computed tomographies (CBCT) in orthodontics and pediatric dentistry is constantly increasing. The aim of this systematic review was to critically appraise and summarize evidence from clinical studies on the prevalence of incidental findings from CBCTs of children and adolescents.

Methods: Systematic literature searches without restrictions were undertaken in eight databases from inception up to March 2024 for studies reporting on incidental findings from CBCT images of children and adolescents.

Quaternary Ammonium Compounds: A New Driver and Hidden Threat for Prevalence in Hospital Wastewater and Human Feces.

The emergence of mobile colistin resistance gene has attracted global attention. The prevalence of -positive (MCRPEC) in humans largely decreased following the ban of colistin as an animal growth promoter in China. However, the prevalence of MCRPEC in the hospital environment and the relationship between disinfectants and remain unclear.