Circulating microRNAs in Medicine.

Circulating microRNAs (c-microRNAs, c-miRNAs), which are present in almost all biological fluids, are promising sensitive biomarkers for various diseases (oncological and cardiovascular diseases, neurodegenerative pathologies, etc.), and their signatures accurately reflect the state of the body. Studies of the expression of microRNA markers show that they can enable a wide range of diseases to be diagnosed before clinical symptoms are manifested, and they can help to assess a patient's response to therapy in order to correct and personalize treatments.

Inhibition of fibrin polymerization by synthetic peptides corresponding to Aα195-205 and γ69-77 sites of fibrin molecule.

Using the idea of "proline brackets" we have found four sites in fibrin amino acid sequence, and appropriate peptides were synthesized: γ69NPDESSKPN77, Bβ228QPDSSVKPY236, Bβ455RPFFPQ460 and Aα195LPSRDRQHLPL205. Turbidity and electron-microscopy analyses have demonstrated that synthetic peptide Aα195-205 specifically inhibited the stage of fibrin protofibril formation and peptide γ69-77 - the stage offibrin protofibril lateral association. The data obtained testify that there are the sites involved in these processes in the appropriate amino acid sequences of fibrin molecule.

[Distribution of the total quantity of fatty acid double bonds in lipid components of the blood in children with diabetes mellitus].

Children suffering from insulin-dependent diabetes mellitus (IDDM) were examined for unsaturation, that is, total quantity of double bonds in individual fractions of blood serum lipids was assessed. Lipid fractions were isolated by thin-layer chromatography. Unsaturation was assessed by ozonation method.

[The indices of the lipid peroxidation and desaturation of the blood serum in diabetes mellitus in children].

Comparative investigation of indices of lipid peroxidation and blood lipid unsaturation was carried out in 91 pediatric inpatients (aged 7 to 15) with type I diabetes mellitus and in 10 controls of the same age. Activation of free radical lipid peroxidation was shown to occur in children with diabetes mellitus. A degree of compensation of diabetes did not influence the level of hydroxyperoxides (HP) and final decay products (FP) of lipid peroxidation.

[Study of unsaturated plasma lipids in children with diabetes mellitus].

The determination of unsaturation of lipids contained by the blood serum and cell plasma membranes, which depends on the total amount of double bonds (DB) in unsaturated fatty acids is a new and promising method of estimating lipid metabolism in diabetes mellitus. Unsaturation was determined by ozonization. 91 children aged 7 to 15 years were examined.