Effect of Surface Modification of Gold Nanoparticles Loaded with Small Nucleic Acid Sequences on Cytotoxicity and Uptake: A Comparative Study In Vitro.

Nanoparticle technology, particularly gold nanoparticles (AuNPs), is being developed for a wide range of applications, including as a delivery system of peptides or nucleic acids (NA). Their use in precision medicine requires detailed engineering of NP functionalization to optimize their function and minimize off-target toxicity. Two main routes can be found in the literature for the attachment of NA strands to AuNPs: covalent binding via a thiol group or passive adsorption onto a specially adapted coating previously applied to the metallic core.

Economic returns on investing in early childhood development in Vietnam: a cost-benefit analysis.

Background: Economic evidence on the long-term benefits of investing in early childhood development is limited. This study aimed to estimate the potential long-term economic benefits of an early childhood development intervention 'Learning Clubs' in Vietnam.

Methods: We conducted a cost-benefit analysis to estimate the costs and benefits of the intervention compared to the standard of care from a limited societal perspective.

Cholesterol Sulfate as a Negative Regulator of Cellular Cholesterol Homeostasis.

Cholesterol sulfate (CS), one of the most abundant cholesterol derivatives, recently emerged as a key regulatory molecule in several physiological processes. Here, we demonstrate multiple mechanisms by which CS reduces intracellular cholesterol levels. CS promotes the proteasomal degradation of HMG-CoA reductase (HMGCR) by enhancing INSIG-mediated ubiquitination, thereby inhibiting cholesterol synthesis.

Laparoscopic surgery for impacted dentures in the descending colon: A case report.

Introduction And Importance: Denture ingestion, commonly seen in older adults, can also occur in younger individuals. Most dentures require intervention as they cannot be excreted naturally. We report a case of a young male undergoing laparoscopic surgery to remove dentures impacted in the descending colon two months post-ingestion.

Probabilistic analysis of active earth pressures in spatially variable soils using machine learning and confidence intervals.

This study introduces a probabilistic framework for assessing active earth pressures in soils exhibiting spatial variability in friction angles and unit weight. These properties are modeled using random fields with log-normal distributions and spatial correlation lengths. Monte Carlo simulations (MCS) are integrated with finite element limit analysis (FELA) to evaluate the failure probability under different design safety factors.