[Modelling of insulin resistance and associated metabolic disorders using dexamethasone].

In experiment carried out on 12 rabbits, the possibility of reproduction of insulin resistance syndrome components in the absence of obesity as initiating factor was assessed. The experimental rabbits were subjected to subcutaneous injections of synthetic glucocorticoid dexametazone during 8 weeks in the dose 15 mkg/kg. The obtained data showed a progressive decline in insulin sensitivity in the course of the experiment, and at the end of the 8-th week the reaction to subcutaneous insulin injection decreased by 82%, the content of glycosilated hemoglobin increased by 200%.

[Hypertrygliceridemia as a factor of atherogenesis: significance and mechanisms of action].

The aim of the study was to investigate the pattern of lipid and lipoprotein metabolism disturbances in patients with manifested clinical signs of coronary atherosclerosis. There was determined the significance of hypertrigliceridemia as the marker of these disturbances and its connection with other most important factors of atherogenesis such as insulin resistance, systemic inflammation, and oxidative stress, blood lipoprotein modification. The role of excessive alimentary lipid consumption as a factor, which initiates the hypertrigliceridemia development and accompanying activation of the other proatherogenic factors was determined with the use of acute lipid loading.

[The presence and characteristics of interrelations between lipid metabolism disorder and systemic inflammation].

The aim of the investigation was to determine the existence and describe interconnection between the lipid metabolism disturbances and development of systemic inflammation. The work was carried out on rabbits in two series of experiments: in the first one, the model of inflammation was reproduced by means of intravenous injection of bacterial lipopolysaccharide. In the second one, the model of lipid disturbances induced by lipids enriched diet was reproduced.

[Hyperlipidemia and decrease of lipid tolerance as factors of atherogenesis].

In experiments performed on rabbits the influence of acute and chronic alimentary lipid loading on the pathogenic factors of atherosclerosis was examined. A single moderate lipid loading even in normal rabbits was established to induce a transitory development of the proatherogenic changes, which includes appearance of dislipidemia of diabetic type, activation of systemic inflammation and free radical reactions, blood lipoprotein modification. Prolonged keeping of rabbits on the diet enriched with lipids induced the development of complex changes which are typical for insulin resistance with specific lipid and lipoprotein metabolism disturbances, increased plasma atherogenic potential, activation of systemic inflammation and oxidative stress.